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The permeability of the leak pathway can be better assessed ex vivo by measuring the flux of molecules from the luminal to the basolateral side such as EDTA, mannitol, sucrose, inulin and polyethylene glycols (PEG) or dextran molecules of variable sizes (ranging from 4 up to 20 KDa)

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Retinol (Vitamin A) and vitamin C promote collagen synthesis indirectly retinol via retinoic acid receptor signaling, vitamin C as a co-factor in collagen cross-linking

A prospective, randomized, placebo controlled, double-blind study of amitriptyline for the treatment of interstitial cystitis